WebLuria–Bertani (LB) broth (10.0 g tryptone l 21, 5.0 g yeast extract l and 5.0 g NaCl l 21 ) and agar (LB broth supplemented with 15 g agar l 21 ) were used to maintain all bacterial strains. Web2016), and grown in Neurobasal Medium (Gibco), containing NEAA, L-Glutamine, penicillin/ streptomycin, B27, FGF2, and LIF. Cells were cultured at 37°C in 5% CO 2 and 8% O 2. Total ... Separations of the tryptic peptides from digested samples and sample fractions were performed on a NanoLC™ 425 System (SCIEX) in trap elute mode, using a 75
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WebThe spontaneously immortalized HaCaT human keratinocyte cell line was cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco ®) with 10% fetal calf serum (FCS; Gibco ®) as previously described by Boukamp et al. 29 PAR-2 was first activated in HaCaT keratinocytes by stratum corneum tryptic enzyme (SCTE; R&D Systems), leading to … Web1 ISSN 1062-3590, Biology Bulletin, 2024. © Pleiades Publishing, Inc., 2024. Janibacter sp. Isolated from Deschampsia antarctica Rhizosphere as a Potential Source of ... chuckling cheese hampers
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