Phosphinothricin-resistant

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August undefined, 2024

WebResistance plasmids include pGreenII 0229 and pGreenII 0229 62-SK. pGreenII 0229 is derived from pGreenII 0000, a nos-bar cassette has been inserted into the HpaI site of the left border, providing resistance to bialaphos or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK is derived from pGreenII 0229, the LacZ … WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ...

(PDF) The use of phosphinothricin resistance as ... - ResearchGate

WebCAS No. 7803-51-2. Phosphine (PH₃) is a colorless, flammable, and explosive gas at room … WebSep 12, 2015 · In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus reporter and a bar … cindy\u0027s secret place

Use of bar as a selectable marker gene and for the production of ...

WebA gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. WebPhosphinothricin C5H11NO4P- CID 25244585 - structure, chemical names, physical and … diabetic ketoacidosis fruity odor

Establishment of a chloroplast transformation system in

Non-specific activities of the major herbicide-resistance …

WebJul 14, 2024 · In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 and ant2) driven by the endogenous psbA and rbcL promoters were cloned in this vector. The recombinant plasmid was transformed using a … WebPhosphinothricin, as a glutamic acid analog, irreversibly inhibits glutamine synthetase (EC 6.3.1.2) leading to a decrease in the amino acids glutamine, aspartate, serine, glycine, and alanine in the affected organism. The herbicidal effect can be reversed by glutamine (see Bayer et al., 1972 ). cindy\\u0027s secret gardenWebJan 1, 2008 · Phosphinothricin resistance as selectable marker for genetic transformation … diabetic ketoacidosis high amino acids

"WebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces hygroscopicus, has an enzyme that inactivates phosphinothricin and glufosinate by acylating it. The gene (bar) encoding this... " - Phosphinothricin-resistant

Phosphinothricin-resistant

WebPhosphinothricin. Resistance against PTT can be achieved by elevated levels of GS, which … Webresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the

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WebNov 6, 2006 · The phosphinothricin-resistant seedlings were uniformly much greener than … WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1.

WebOct 4, 2009 · The transgenic plants were resistant to phosphinothricin up to 200 mg/l, … WebA bioassay was developed for identifying transgenic wheat plants based on De Block et …

WebPhosphinothricin is not an effective antibiotic because of its inability to be readily … WebPlant resistance to phosphinothricin is conferred through the bar gene, and due to the fast degradation of phosphinothricin in soil and in water, it presents a low risk for environmental contamination. Product Specifications Phosphinothricin Glufosinate or Glufosinate ammonium Also known as Bayer’s Basta TM Formula: C 5 H 15 N 2 O 4 P

WebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine …

WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic . transformation of grapevine. R. J ARDAK-J AMOUSSI 1), B. B OUAMAMA 1), A. M LIKI 1), A. G HORBEL 1) and G. M. R EUSTLE 2) cindy\\u0027s school of dance allenWebNov 6, 2006 · Selection for kanamycin-, phosphinothricin- and hygromycin B-resistance commonly takes 7-10 d and high seedling density and fungal contamination may result in failure to recover transformants. Results: A method for identifying transformed seedlings in as little as 3.25 d has been developed. cindy\u0027s sewing and embroideryGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring herbicides, directly into glufosinate. The … cindy\u0027s sfWebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) carfentrazone-ethyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions … cindy\\u0027s sfWebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... diabetic ketoacidosis gestational diabetesWebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces … diabetic ketoacidosis emergency room cindy\\u0027s sewing and embroidery