WebFluorescein-CE Phosphoramidite has a thiourea linkage which mimics the original method of incorporating fluorescein to an amino-modified oligo. It must be noted however that the linkage is attached via the 5 position of the ring system in this case. Internal sequence additions of Fluorescein are achieved using Fluorescein-dT. WebThere are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5'-fluorescein-CE phosphoramidite, derived from the single isomer 6-FAM, 6-HEX, 6-TET and 6-JOE, can all be used to efficiently label an oligonucleotide at the 5'-end.
6-JOE Phosphoramidite AAT Bioquest
WebThere are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5'-fluorescein-CE phosphoramidite, derived from the single isomer 6-FAM, 6-HEX, 6-TET and 6-JOE, can all be used to efficiently label an oligonucleotide at the 5'-end. Web6-FAM phosphoramidite is an oligonucleotide label. There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5′-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5′-hexachlorofluorescein-CE phosphoramidite ... gpu with no display out
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WebFluorescein phosphoramidite is designed to produce the same fluorescein-type structure as had been previously prepared using fluorescein isothiocyanate (FITC). Our fluorescein … WebThere are also two phosphoramidite monomers that can be used for oligonucleotide fluorescein labeling: 6-Fluorescein-CE Phosphoramidite and Fluorescein-CE Phosphoramidite, both of which contain the same fluorescent dye as 6-FAM CE Phosphoramidite, but with different linkage backbones. Web5’-Biotin-CE Phosphoramidite (LK2109) – A 5min coupling time is recommended. The trityl group is slow to detritylate, therefore removal on the synthesiser requires an additional deblocking step. If the biotin DMTr group is retained to aid cartridge purification, we recommend that the oligo is left in contact with the TFA solution for 10min. gpu with 6 pin connector