Fk2 antibody

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August undefined, 2024

WebMar 25, 2016 · We previously developed an FK2 antibody, which specifically recognizes poly-Ub moie … Polyubiquitination plays key roles in proteasome-dependent and independent cellular events, whereas monoubiquitination is involved in gene expression, DNA repair, protein-protein interaction, and protein trafficking. WebWestern blots were developed using PVDF membranes and A-106 anti-ubiquitin primary antibody at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) …

Anti-Ubiquitinylated proteins Antibody, clone FK1 04-262

WebUbiquitin Antibody - Mouse Monoclonal antibody to Ubiquitin, FK2. Species Independent. Validated for use in Western Blot, IHC, ICC/IF, IP, and ELISA. WebThe FK2 antibody works great in both human and rat samples (I haven't tried mouse) by western blot and immunofluorescence. This antibody should recognize the ubiquitin modifications (both mono... list of lawyers in mumbai

Anti-Ubiquitin Mouse mAb (FK2) ST1200

Web11 rows · Dilute to working strength with phosphate buffered saline pH 7.2-7.4. This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use … WebNational Center for Biotechnology Information WebJan 7, 2024 · The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterized by one-dimensional Western blotting. The immunoglobulin … list of lawyers in melbourne

(BML-PW8810-0500) Mono- and polyubiquitinylated conjugates

Ubiquitinylated proteins Mouse anti-All, Clone: FK2, …

WebMay 15, 2005 · Separation of ubiquitinated Syk using an antiubiquitin antibody. Washed platelets were activated with convulxin (100 ng/mL) for 15 seconds. Cells were lysed with RIPA buffer and treated with FK2 antibody for 1 hour at 4°C, followed by Protein A/G PLUS-agarose for an additional hour. Supernatants were treated with anti-Syk using the … WebMar 20, 2024 · Ubiquitin staining was performed using anti-ubiquitin (FK2) antibody. The blue (Hoescht 33342) but not red bacteria are L. dumoffii. Ubiquitin was commonly associated with LpCVs (arrows), but was ... list of lawyers in netherlandsWeb11 rows · Anti-Ubiquitinylated proteins Antibody, clone FK2. Background Information. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a … imdb actress 1.75m born in 1993

"WebDownload Zoom This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References Data Sheet … " - Fk2 antibody

Fk2 antibody

WebApr 14, 2024 · Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) 单泛素/多泛素偶联物单抗（FK2）品牌：Enzo; 13HP020AN-东洋一次性针头式过滤器0.2um孔径; 月桂硫酸盐肉汤培养基-其它; Whatman 滤膜 6720-5001 盘式滤器 Polydisc TF, 0.1m 10/盒 WebFeb 21, 2014 · The third method utilized the anti-ubiquitin antibody FK2. In the initial studies, the FK2 method was the most efficient method of isolating endogenously ubiquitinated protein complexes and recognizing monoubiquitinated and …

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WebAnti-Ubiquitinylated proteins Antibody, clone FK1 04-262 Sigma-Aldrich Anti-Ubiquitinylated proteins Antibody, clone FK1 Download Zoom Anti-Ubiquitinylated proteins Antibody, clone FK1 is an antibody against … WebFeb 11, 2024 · Anti-Multi Ubiquitin mAb(Monoclonal, FK2 from MBL.Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono …

WebAug 17, 2011 · The FK1 and FK2 monoclonal antibodies are used to differentiate between monoubiquitination and polyubiquitination, since FK2 detects both monoubiquitinated and polyubiquitinated proteins, while FK1 recognizes only polyubiquitinated proteins (Fujimuro et al., 1994). Therefore, to assess whether the ubiquitination of PSD-95 reflects ... WebUbiquitin modification of p62 was detected using an antibody recognizing mono- and poly-ubiquitin (FK2) (lower panels). The EGFP–TRIM proteins were detected using an anti-GFP antibody. * indicates mono-ubiquitylated p62 and a high-molecular-mass p62.

WebMar 23, 2024 · The membranes were afterward incubated with mouse anti-phosphotyrosine antibody (clone 4G10; Millipore, MA, USA) diluted 1:1,000 in PBS or mouse anti-ubiquitin antibody (FK2, 1:250 dilution, monoclonal mouse antibody recognizing mono- and polyubiquitinated conjugates; ENZO Life Sciences, NY, USA) at 4°C overnight. WebSep 10, 2016 · Scheme of the ELISA assay. (a) In the “forward” approach, microwell plates are coated with a polyclonal anti-EGFR intra, which captures the receptor from the lysate (1).Detection of Ub-EGFR or total EGFR (to normalize) is performed with primary monoclonal antibodies directed against Ub (FK2, 1) or EGFR extra (2), respectively, …

WebThis Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting …

WebJan 7, 2024 · antibody product name : Polyubiquitinylated conjugates monoclonal antibody (FK1) catalog : BML-PW8805-0500 quantity : 500 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK1 reactivity : spiny dogfish, human, mouse, rat, fruit fly application : western blot, ELISA, immunohistochemistry, immunocytochemistry, … list of lawyers in los angelesWebFeb 11, 2024 · Feb 11, 2024 in JST） Size 100 µg/100 µL Citations Data Sheet SDS Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) . The availability is based on the information in Japan at 00:05, Feb 11, 2024 in JST. imdb actors from texasWebJan 7, 2024 · product name : Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) catalog : BML-PW8810-0100 quantity : 100 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK2 reactivity : southern flounder, Saccharomycetales, human, mouse, rat, fruit fly application : imdb actors from virginiaWebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin … list of lawyers in ohioWebSep 15, 2013 · The FK2 antibody is a mouse monoclonal recognizing both monoubiquitinated and polyubiquitinated conjugates. According to the manufacturer’s data sheet the FK2 antibody can recognize various types of ubiquitin chains; however, it is unknown if it has equal affinities for all seven ubiquitin chain linkages and for different … imdb actors in their 30sWebProperties Biological Description Product Images Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-550). Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 µg. imdb actressWebBlock: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody (SMC-550) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti … imdb actress 1.75m 9-1-1